1)         Binding Rate formula: (Sample O.D.÷Positive Control O.D.)×100%.
2)         Notes for vaccine: the IgM will gradually disappear after 60 days of vaccine.
3)         The animal source is healthy: Binding rate of the samples is＜70%.
4)         The animal source is under infection: Binding rate of the samples is≥80%.
5)         The animal source is suspected of being infected: Binding rate is 70%~80%.

1)        Sensitivity: ＞95%.
2)        Specificity: there is no cross-reaction with other substances in the samples.
3)        Security: Pre-coated antigen, HRP-conjugated antigen and controls only have immunogenicity, non-microorganism toxicity and infectivity.
4)        Intra-assay: CV＜10% (n=3).
5)        Inter- assay: CV＜15% (n=9).
6)        The experimental results should meet the following conditions. Should any of these criteria not be fulfilled, the test is invalid and the assay should be repeated: 
Mean OD450nm of Positive control＞0.50.