1)    Block Rate formula: [(Negative O.D.➖Sample O.D.)÷Negative O.D.]×100%;

2)    Total antibody Negative: Block rate is＜40%, the immunization is unqualified.
3)    Total antibody Positive: Block rate is≥40%, the immunization is qualified.

1)    Sensitivity: 100%
2)    Specificity: there is no cross-reaction with other substances in the samples.
3)    Security: Pre-coated antigen, HRP-conjugated antibodies and controls only have immunogenicity, non-microorganism toxicity and infectivity.
4)    Intra-assay: CV＜ 10% (n=3).
5)    Inter- assay: CV＜ 15% (n=9).
6)    The experimental results should meet the following condition. Should any of these criteria not be fulfilled, the test is invalid and the assay should be repeated.: Block rate of Positive control＞40%.