Bovine viral diarrhea (BVD) or bovine viral diarrhoea , and previously referred to as bovine virus diarrhoea (BVD), is a significant economic disease of cattle that is found in the majority of countries throughout the world. Worldwide reviews of the economically assessed production losses and intervention programs (e.g. eradication programs, vaccination strategies and biosecurity measures) incurred by BVD infection have been published. The causative agent, bovine viral diarrhea virus (BVDV), is a member of the genus Pestivirus of the family Flaviviridae.
1) Negative: Samples’ O.D. ≤ Negative Control’s OD450nm , then the animal source is healthy.
2) Positive: Samples’ O.D. ＞Negative Control’s OD450nm , then the animal source is infected.
3) Positive: Samples’ O.D. (90 days after vaccine) ﹥Negative Control’s OD450nm , then the animal source is infected.
1) Sensitivity: 100%
2) Specificity: there is no cross-reaction with other substances in the samples.
3) Security: Pre-coated antigen, HRP-conjugated antibodies and controls only have immunogenicity, non-microorganism toxicity and infectivity.
4) Intra-assay: CV＜10% (n=3).
5) Inter- assay: CV＜15% (n=9).
6) Assay established condition: Positive Control’s OD450nm＞1.0.
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