Datasheet
别称
S. cerevisiae Host Cell Protein ,G3 ELISA Kit
S. cerevisiaeHCP;S. cerevisiae HCP
研究领域
定量检测培养上清、蛋白纯化过程及终产物中宿主细胞蛋白残留
背景介绍
本试剂盒是用来定量分析以酿酒酵母（Saccharomycescerevisiae）为表达系统时，蛋 白药物中宿主细胞残留蛋白。 酿酒酵母作为常用的表达系统在表达目的蛋白的同时，会伴随着表达细胞自身凋亡，资 料显示细胞破碎后释放到培养基中的宿主蛋白达上千种之多，其中很大一部分具有很强的免 疫原性，导致不良的毒性或免疫反应而危及产品安全和质量，造成潜在的生物污染，生物医 药产品生产下游过程的目的之一就是移除这些潜在危害。 因此，非常有必要将宿主细胞蛋白（HCP）残留量降低到最低水平，在研发下游纯化的 工艺时，必须具有一种科学合理的测定成品或者半成品中HCP浓度的方法，而酶联免疫法具 有极高灵敏度，因而被FDA,EMA,CFDA及ICH等国内外监管机构定为HCP检测的金标准。
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<span style="color:#0066cc;"><b><span arial="" lang="EN-US" unicode="">名称：</span></b></span></p>
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<span><span><span><span>CHO HCP G3</span></span></span></span><span arial="" lang="EN-US" style="font-family: " unicode=""> ELISA<o:p></o:p></span></p>
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<span><b style="color: rgb(0, 102, 204); background-color: rgb(247, 247, 247);"><span arial="" lang="EN-US" unicode="">规格：</span></b></span></p>
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<span><span style="background-color: rgb(247, 247, 247);">96/48 Tests</span></span></p>
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<span><b style="color: rgb(0, 102, 204);"><span arial="" lang="EN-US" unicode="">浓度范围：</span></b></span></p>
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<span>0-400 ng/mL</span></td>
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<span><b style="color: rgb(0, 102, 204); background-color: rgb(247, 247, 247);"><span arial="" lang="EN-US" unicode="">最低检测限：</span></b></span></p>
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<span><span style="background-color: rgb(247, 247, 247);">0.78ng/mL</span></span></td>
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<span><b style="color: rgb(0, 102, 204);"><span arial="" lang="EN-US" unicode="">检测方法：</span></b></span></p>
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酶联免疫<span>夹心法</span></td>
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<span><b style="color: rgb(0, 102, 204); background-color: rgb(247, 247, 247);"><span arial="" lang="EN-US" unicode="">加样量：</span></b></span></p>
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<span><span style="background-color: rgb(247, 247, 247);">100 ul </span></span></td>
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<span><b style="color: rgb(0, 102, 204);"><span arial="" lang="EN-US" unicode="">实验时间：</span></b></span></p>
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<span>3.5-4 h</span></td>
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<span><b style="color: rgb(0, 102, 204);"><span arial="" lang="EN-US" unicode="">酶结合物：</span></b></span></p>
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<span>HRP</span></td>
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<span style="color:#0066cc;"><b><span arial="" lang="EN-US" unicode="">仪器：</span></b></span></p>
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<span arial="" lang="EN-US" style="font-family: " unicode="">酶标仪<o:p></o:p></span></p>
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<span><b style="color: rgb(0, 102, 204);"><span arial="" lang="EN-US" unicode="">储存条件：</span></b></span></p>
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<span>2-8°C</span></td>
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|
名称： |
S. cerevisiae HCP G3 ELISA |
|
规格： |
96/48 Tests |
|
浓度范围： |
0-800 ng/mL |
|
最低检测限： |
0.39ng/mL |
|
检测方法： |
酶联免疫夹心法 |
|
加样量： |
100ul |
|
实验时间： |
3.5-4 h |
|
酶结合物： |
HRP |
|
仪器： |
酶标仪 |
|
储存条件： |
2-8°C |
|
|
四参数Logistic曲线拟合
==================================================================
方程: y = (A - D) / [1 + (x/C)^B] + D
------------------------------------------------------------------
A = 4.86052
B = -1.06393
C = 912.97478
D = 0.00762
r^2 = 0.99995
==================================================================
==================================================================
X Y-反应值 Y-平均值 CV(%) Y-计算值 Y-残差
------------------------------------------------------------------
6.2500 0.0335 0.0317 -0.0018
25.0000 0.1145 0.1110 -0.0035
50.0000 0.2100 0.2188 0.0088
100.0000 0.4280 0.4290 0.0010
200.0000 0.8210 0.8124 -0.0086
400.0000 1.4270 1.4324 0.0054
800.0000 2.2650 2.2638 -0.0012
==================================================================
数据个数 7
残差平方和 0.00020
四参数Logistic曲线拟合
==================================================================
方程: y = (A - D) / [1 + (x/C)^B] + D
------------------------------------------------------------------
A = 4.86052
B = -1.06393
C = 912.97478
D = 0.00762
r^2 = 0.99995
==================================================================
==================================================================
X Y-反应值 Y-平均值 CV(%) Y-计算值 Y-残差
------------------------------------------------------------------
6.2500 0.0335 0.0317 -0.0018
25.0000 0.1145 0.1110 -0.0035
50.0000 0.2100 0.2188 0.0088
100.0000 0.4280 0.4290 0.0010
200.0000 0.8210 0.8124 -0.0086
400.0000 1.4270 1.4324 0.0054
800.0000 2.2650 2.2638 -0.0012
==================================================================
数据个数 7
残差平方和 0.00020
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