Datasheet
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蛋白及NCBI编号： |
NM_001963.6 |
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宿主： |
E.coli | ||
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表达区域： |
Asn971-Arg1023 |
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别名： |
Pro-epidermal growth factor，EGF |
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蛋白序列： |
NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGER CQYRDLKWWELR |
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分子量： |
蛋白由53个氨基酸组成，预测分子量为6.2kDa，实际分子量与预测一致 |
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融合标签： |
无 | ||
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纯度： |
≥95% 蛋白电泳 | ||
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物理性状： |
液态 | ||
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组分： |
0.01M PBS+20%甘油，溶液无菌 | ||
| 稳定性： 分装后样品在-20℃至-80℃下的稳定性可达6个月，避免反复冻融 | |||
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应用： 抗体制备，免疫实验（ELISA，WB），亚细胞定位和互作蛋白鉴定等。 |
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内毒素： < 0.1 EU per ug of the protein as determined by the LAL method |
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别名
Pro-epidermal growth factor; EGF.
背景
人表皮生长因子（EGF）是6-kDa的蛋白质，具有53个氨基酸残基和三个分子内二硫键，通过与细胞表面的同源受体EGFR结合，可以刺激细胞生长、分化和存活。EGF在体内和体外刺激各种表皮和上皮组织的生长，在细胞培养中刺激一些成纤维细胞的生长。这刺激配体诱导的二聚化，激活受体的固有蛋白酪氨酸激酶活性。
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