SDS
Other Names
5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone
Research Area
Metabolism
Background
Dihydrotestosterone is also known as 17beta-hydroxy-5alpha-androstane-3-one, 5alpha-dihydrotestosterone, 5alpha-DHT, or stanorone. It is a sterol whose double bond of testosterone is reduced at 5a. It acts as a true androgen to the accessory glands (prostate, seminal vesicle, etc.). The 5a-hydrogenase that forms the sterol exists in the cells of the accessory gonads. The complex of sterol and receptor proteins bound in cells enters the nucleus of the adnexal gland and promotes the synthesis of new proteins. It showed a slightly stronger androgenic effect than testosterone. Although 5beta-body exists in the form of testosterone in liver and chicken crown, it has no effect of male hormone. 5alpha-dihydrotestosterone is metabolized to 5alpha-androstane-3alpha-17beta-diol by the action of 3alpha-hydroxysterol dehydrogenase and excreted in vitro.
|
Product Name |
DHT ELISA |
|
Species |
Monkey |
|
Product Size |
96/48 Tests |
|
Concentration |
100-2500 pg/mL |
|
Sensitivity |
16.4 pg/ml |
|
Principle |
Competitive ELISA |
|
Sample Volume |
100 ul |
|
Assay Time |
90 minutes |
|
Platform |
Microplate Reader |
|
Conjugate |
HRP |
|
Detection Method |
Colorimetric |
|
Storage |
2-8°C |
|
|
For Research use only
Formular: y = (A - D) / [1 + (x/C)^B] + D
A = 1.77782 B = 1.62110 C = 303.27773 D = 0.04416 r^2 = 0.99777 |
1. Protocols for ELISA
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1) Direct ELISA
2) Direct ELISA Using Fluorescent Substrate
3) Indirect ELISA
4) Sandwich ELISA
2. Protocols for IHC ICC
1) Determining if the antibody binds only phosphorylated protein (WB or IHC)
2) Double immunofluorescence-sequential protocol
3) Double immunofluorescence-simultaneous protocol
4) Fixation and Permeabilization In IHC ICC
5) Glycol Methalacrylate Acrylic Resin Embedding For IHC
9) Immunohistochemistry (IHC-Fr) - Frozen Sections
3. Protocols for WB
4) S-100 Mitochondrial Fractionation
5) Stripping for Reprobing Western Blots
7) Western Blotting - A Beginner's Guide
8) Western Blotting of Phospho-Proteins
9) Western Blotting Using Antibodies Against Histone Proteins
4. Protocols for IP
2) Using IgM antibodies for IP
5. Protocols for FACS
1) Direct Staining Protocol (Cell Surface Staining)
3) Flow Cytometry Whole Blood Samples-Red Blood Cell Lysis
4) Indirect Staining Protocol (Cell Surface Staining)
6) Recommended Controls for FACS
6. Protocols for ELISPOT
1) ELISPOT
1. Protocols for ELISA
1) Direct ELISA
2) Direct ELISA Using Fluorescent Substrate
3) Indirect ELISA
4) Sandwich ELISA
2. Protocols for IHC ICC
1) Determining if the antibody binds only phosphorylated protein (WB or IHC)
2) Double immunofluorescence-sequential protocol
3) Double immunofluorescence-simultaneous protocol
4) Fixation and Permeabilization In IHC ICC
5) Glycol Methalacrylate Acrylic Resin Embedding For IHC
9) Immunohistochemistry (IHC-Fr) - Frozen Sections
3. Protocols for WB
4) S-100 Mitochondrial Fractionation
5) Stripping for Reprobing Western Blots
7) Western Blotting - A Beginner's Guide
8) Western Blotting of Phospho-Proteins
9) Western Blotting Using Antibodies Against Histone Proteins
4. Protocols for IP
2) Using IgM antibodies for IP
5. Protocols for FACS
1) Direct Staining Protocol (Cell Surface Staining)
3) Flow Cytometry Whole Blood Samples-Red Blood Cell Lysis
4) Indirect Staining Protocol (Cell Surface Staining)
6) Recommended Controls for FACS
6. Protocols for ELISPOT
1) ELISPOT
