SDS
Other Names
HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1
Research Area
Immunology, Microbiology
Background
Traditional blood and urine markers for the diagnosis of various renal diseases are insensitive and nonspecific. Kidney Injury Molecule-1 (KIM-1) is a type 1 transmembrane protein, with an immunoglobulin and mucin domain, whose expression is markedly up-regulated in the proximal tubule in the post-ischemic rat kidney. The ectodomain of KIM-1 is shed from cells. The current studies were carried out to evaluate whether KIM-1 is present in human acute renal failure and might serve as a urinary marker of acute renal tubular injury.
|
Product Name |
KIM1ELISA |
|
Species |
Monkey |
|
Product Size |
96/48 Tests |
|
Concentration |
1.0-25 ng/ml |
|
Sensitivity |
0.09 ng/ml |
|
Principle |
Competitive ELISA |
|
Sample Volume |
100 ul |
|
Assay Time |
90 minutes |
|
Platform |
Microplate Reader |
|
Conjugate |
HRP |
|
Detection Method |
Colorimetric |
|
Storage |
2-8°C |
|
|
For Research use only
Formular: y = (A - D) / [1 + (x/C)^B] + D
A = 2.45541 B = 1.23695 C = 3.76072 D = 0.38392 r^2 = 0.99960 |
1. Protocols for ELISA
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1) Direct ELISA
2) Direct ELISA Using Fluorescent Substrate
3) Indirect ELISA
4) Sandwich ELISA
2. Protocols for IHC ICC
1) Determining if the antibody binds only phosphorylated protein (WB or IHC)
2) Double immunofluorescence-sequential protocol
3) Double immunofluorescence-simultaneous protocol
4) Fixation and Permeabilization In IHC ICC
5) Glycol Methalacrylate Acrylic Resin Embedding For IHC
9) Immunohistochemistry (IHC-Fr) - Frozen Sections
3. Protocols for WB
4) S-100 Mitochondrial Fractionation
5) Stripping for Reprobing Western Blots
7) Western Blotting - A Beginner's Guide
8) Western Blotting of Phospho-Proteins
9) Western Blotting Using Antibodies Against Histone Proteins
4. Protocols for IP
2) Using IgM antibodies for IP
5. Protocols for FACS
1) Direct Staining Protocol (Cell Surface Staining)
3) Flow Cytometry Whole Blood Samples-Red Blood Cell Lysis
4) Indirect Staining Protocol (Cell Surface Staining)
6) Recommended Controls for FACS
6. Protocols for ELISPOT
1) ELISPOT
1. Protocols for ELISA
1) Direct ELISA
2) Direct ELISA Using Fluorescent Substrate
3) Indirect ELISA
4) Sandwich ELISA
2. Protocols for IHC ICC
1) Determining if the antibody binds only phosphorylated protein (WB or IHC)
2) Double immunofluorescence-sequential protocol
3) Double immunofluorescence-simultaneous protocol
4) Fixation and Permeabilization In IHC ICC
5) Glycol Methalacrylate Acrylic Resin Embedding For IHC
9) Immunohistochemistry (IHC-Fr) - Frozen Sections
3. Protocols for WB
4) S-100 Mitochondrial Fractionation
5) Stripping for Reprobing Western Blots
7) Western Blotting - A Beginner's Guide
8) Western Blotting of Phospho-Proteins
9) Western Blotting Using Antibodies Against Histone Proteins
4. Protocols for IP
2) Using IgM antibodies for IP
5. Protocols for FACS
1) Direct Staining Protocol (Cell Surface Staining)
3) Flow Cytometry Whole Blood Samples-Red Blood Cell Lysis
4) Indirect Staining Protocol (Cell Surface Staining)
6) Recommended Controls for FACS
6. Protocols for ELISPOT
1) ELISPOT
