SDS
Other Names
Canine 2,3 Diphosphoglycerate ELISA kit
2,3BPG; 2,3-DPG; 2,3-Diphosphoglycerate
Research Area
Metabolism
Background
The binding of 2,3-DPG to deoxyhemoglobin in red blood cells stabilizes the spatial conformation of deoxyhemoglobin, thus reducing the affinity of hemoglobin to oxygen and promoting the dissociation of oxygen and hemoglobin. Especially when blood passes through tissues, the presence of 2,3-DPG in red blood cells can significantly increase the release of O2 for tissue needs. In addition, patients with severe obstructive pulmonary emphysema with pulmonary dyspnea and normal people can adjust the amount of tissue O 2 by changing the concentration of 2,3-DPG in red blood cells when they rise from sea level to high altitude or high altitude in a short time.
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Product Name |
2,3 DPG ELISA |
|
Species |
Canine |
|
Product Size |
96/48 Tests |
|
Concentration |
0.5-10 mmol/mL |
|
Sensitivity |
0.05 mmol/mL |
|
Principle |
Competitive ELISA |
|
Sample Volume |
100 ul |
|
Assay Time |
90 minutes |
|
Platform |
Microplate Reader |
|
Conjugate |
HRP |
|
Detection Method |
Colorimetric |
|
Storage |
2-8°C |
|
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For reference use only.
Four parameter Logisticcurve regression
Formular: y = (A - D) / [1 + (x/C)^B] + D
A = 2.12931
B = 0.94513
C = 3.74840
D = -0.17546
r^2 = 0.99965
|
1. Protocols for ELISA
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1) Direct ELISA
2) Direct ELISA Using Fluorescent Substrate
3) Indirect ELISA
4) Sandwich ELISA
2. Protocols for IHC ICC
1) Determining if the antibody binds only phosphorylated protein (WB or IHC)
2) Double immunofluorescence-sequential protocol
3) Double immunofluorescence-simultaneous protocol
4) Fixation and Permeabilization In IHC ICC
5) Glycol Methalacrylate Acrylic Resin Embedding For IHC
9) Immunohistochemistry (IHC-Fr) - Frozen Sections
3. Protocols for WB
4) S-100 Mitochondrial Fractionation
5) Stripping for Reprobing Western Blots
7) Western Blotting - A Beginner's Guide
8) Western Blotting of Phospho-Proteins
9) Western Blotting Using Antibodies Against Histone Proteins
4. Protocols for IP
2) Using IgM antibodies for IP
5. Protocols for FACS
1) Direct Staining Protocol (Cell Surface Staining)
3) Flow Cytometry Whole Blood Samples-Red Blood Cell Lysis
4) Indirect Staining Protocol (Cell Surface Staining)
6) Recommended Controls for FACS
6. Protocols for ELISPOT
1) ELISPOT
1. Protocols for ELISA
1) Direct ELISA
2) Direct ELISA Using Fluorescent Substrate
3) Indirect ELISA
4) Sandwich ELISA
2. Protocols for IHC ICC
1) Determining if the antibody binds only phosphorylated protein (WB or IHC)
2) Double immunofluorescence-sequential protocol
3) Double immunofluorescence-simultaneous protocol
4) Fixation and Permeabilization In IHC ICC
5) Glycol Methalacrylate Acrylic Resin Embedding For IHC
9) Immunohistochemistry (IHC-Fr) - Frozen Sections
3. Protocols for WB
4) S-100 Mitochondrial Fractionation
5) Stripping for Reprobing Western Blots
7) Western Blotting - A Beginner's Guide
8) Western Blotting of Phospho-Proteins
9) Western Blotting Using Antibodies Against Histone Proteins
4. Protocols for IP
2) Using IgM antibodies for IP
5. Protocols for FACS
1) Direct Staining Protocol (Cell Surface Staining)
3) Flow Cytometry Whole Blood Samples-Red Blood Cell Lysis
4) Indirect Staining Protocol (Cell Surface Staining)
6) Recommended Controls for FACS
6. Protocols for ELISPOT
1) ELISPOT
