SDS
Other Names
Porcine Angiotensin 1 ELISA kit
ANG1；AGP1; AGPT; ANG-I
Research Area
Cardiovascular
Background
Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1.Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability： and may be involved in early development of the heart.
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Product Name |
ANG1 ELISA |
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Species |
Porcine |
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Product Size |
96/48 Tests |
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Concentration |
1.0-25 ng/mL |
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Sensitivity |
0.13 ng/mL |
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Principle |
Competitive ELISA |
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Sample Volume |
100 ul |
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Assay Time |
90 minutes |
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Platform |
Microplate Reader |
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Conjugate |
HRP |
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Detection Method |
Colorimetric |
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Storage |
2-8°C |
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For reference use only.
Four parameter Logisticcurve regression
Formular: y = (A - D) / [1 + (x/C)^B] + D
A = 2.45541
B = 1.23695
C = 3.76072
D = 0.38392
r^2 = 0.99960
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1. Protocols for ELISA
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1) Direct ELISA
2) Direct ELISA Using Fluorescent Substrate
3) Indirect ELISA
4) Sandwich ELISA
2. Protocols for IHC ICC
1) Determining if the antibody binds only phosphorylated protein (WB or IHC)
2) Double immunofluorescence-sequential protocol
3) Double immunofluorescence-simultaneous protocol
4) Fixation and Permeabilization In IHC ICC
5) Glycol Methalacrylate Acrylic Resin Embedding For IHC
9) Immunohistochemistry (IHC-Fr) - Frozen Sections
3. Protocols for WB
4) S-100 Mitochondrial Fractionation
5) Stripping for Reprobing Western Blots
7) Western Blotting - A Beginner's Guide
8) Western Blotting of Phospho-Proteins
9) Western Blotting Using Antibodies Against Histone Proteins
4. Protocols for IP
2) Using IgM antibodies for IP
5. Protocols for FACS
1) Direct Staining Protocol (Cell Surface Staining)
3) Flow Cytometry Whole Blood Samples-Red Blood Cell Lysis
4) Indirect Staining Protocol (Cell Surface Staining)
6) Recommended Controls for FACS
6. Protocols for ELISPOT
1) ELISPOT
