Toxic effect of different ZnO particles on mouse alveolar macrophages
2012-05-301456
abstract
To study the toxicity mechanism of ZnO nanoparticles on mouse macrophages, the toxic effect of differentZnO nanoparticles on mouse alveolar macrophages (MH-S) was investigated in this study. The resultsshowed that the 24 h IC50 of four ZnO particles were 48.53, 47.37, 45.43 and 26.74 g/ml for bulk ZnO,100 nm, 30 nm and 10–30 nm ZnO particles, respectively. At the concentration of 10 g/ml and below,dissolved zinc ions induced metallothionein synthesis, enhanced cellularresistancetooxidativestress.ZnOparticlesmainlyinducedcellapoptosis.Whentheconcentrationof ZnO particles was 20 g/mland above, excessive zinc destroyed mitochondrial function and cell membrane, caused cell necrosis.Dissolved zinc ions first cause toxicity in MH-S cells. However, the toxic effect of dissolved zinc ions mayexist a threshold on mouse macrophages, inducing about 50% cell death. The toxic difference of differentZnO particles mainly depended on the effect of nondissolved ZnO particles.
To study the toxicity mechanism of ZnO nanoparticles on mouse macrophages, the toxic effect of differentZnO nanoparticles on mouse alveolar macrophages (MH-S) was investigated in this study. The resultsshowed that the 24 h IC50 of four ZnO particles were 48.53, 47.37, 45.43 and 26.74 g/ml for bulk ZnO,100 nm, 30 nm and 10–30 nm ZnO particles, respectively. At the concentration of 10 g/ml and below,dissolved zinc ions induced metallothionein synthesis, enhanced cellularresistancetooxidativestress.ZnOparticlesmainlyinducedcellapoptosis.Whentheconcentrationof ZnO particles was 20 g/mland above, excessive zinc destroyed mitochondrial function and cell membrane, caused cell necrosis.Dissolved zinc ions first cause toxicity in MH-S cells. However, the toxic effect of dissolved zinc ions mayexist a threshold on mouse macrophages, inducing about 50% cell death. The toxic difference of differentZnO particles mainly depended on the effect of nondissolved ZnO particles.
