Protective effects of hydrogen-rich saline on monocrotaline-induced pulmonary
2012-01-302027
Abstract
Background
Hydrogen-rich saline has been reported to have antioxidant and anti-inflammatoryeffects and effectively protect against organdamage.Oxidative stress andinflammation contribute to the pathogenesis and/or development of pulmonaryhypertension. In this study, we investigated theeffects of hydrogen-rich saline on theprevention of pulmonary hypertension induced by monocrotaline in a rat model.
Methods
In male Sprague–Dawley rats, pulmonary hypertension was induced by subcutaneousadministration of monocrotaline at a concentration of 6 mg/100 g body weight.Hydrogen-rich saline (5 ml/kg) or saline was administred intraperitoneally once dailyfor 2 or3weeks.Severityofpulmonary hypertension was assessed by hemodynamicindex andhistologicanalysis.Malondialdehydeand8hydroxydesoxyguanosinelevel,andsuperoxide dismutase activity were measured in the lung tissue and serum. Levelsof pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-6) in serum weredetermined with enzyme-linked immunosorbent assay.
Results
Hydrogen-rich saline treatment improved hemodynamics and reverse right ventricularhypertrophy. It also decreased malondialdehyde and 8-hydroxy-desoxyguanosinelevels, and increased superoxide dismutase activity in the lung tissue and serum,accompanied by a decrease inpro-inflammatory cytokines.
Conclusions
These results suggest that hydrogen-rich saline ameliorates the progression ofpulmonary hypertension induced by monocrotaline in rats, which may be associatedwith its antioxidant and anti-inflammatory effects.
Background
Hydrogen-rich saline has been reported to have antioxidant and anti-inflammatoryeffects and effectively protect against organdamage.Oxidative stress andinflammation contribute to the pathogenesis and/or development of pulmonaryhypertension. In this study, we investigated theeffects of hydrogen-rich saline on theprevention of pulmonary hypertension induced by monocrotaline in a rat model.
Methods
In male Sprague–Dawley rats, pulmonary hypertension was induced by subcutaneousadministration of monocrotaline at a concentration of 6 mg/100 g body weight.Hydrogen-rich saline (5 ml/kg) or saline was administred intraperitoneally once dailyfor 2 or3weeks.Severityofpulmonary hypertension was assessed by hemodynamicindex andhistologicanalysis.Malondialdehydeand8hydroxydesoxyguanosinelevel,andsuperoxide dismutase activity were measured in the lung tissue and serum. Levelsof pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-6) in serum weredetermined with enzyme-linked immunosorbent assay.
Results
Hydrogen-rich saline treatment improved hemodynamics and reverse right ventricularhypertrophy. It also decreased malondialdehyde and 8-hydroxy-desoxyguanosinelevels, and increased superoxide dismutase activity in the lung tissue and serum,accompanied by a decrease inpro-inflammatory cytokines.
Conclusions
These results suggest that hydrogen-rich saline ameliorates the progression ofpulmonary hypertension induced by monocrotaline in rats, which may be associatedwith its antioxidant and anti-inflammatory effects.
